Peptide Reconstitution & Dosing Guide

Complete reference for reconstitution, liquid-to-powder math, and common clinical dosing protocols.


The Universal Rule Checklist
  • Always aim the BAC water down the inside glass wall of the vial. Let it dissolve passively. Never shake.
  • Use sterile bacteriostatic water (BAC water) for reconstitution unless otherwise specified.
  • Store reconstituted peptides at 2–8°C and use within 7–14 days.
  • All syringe measurements below assume a standard U-100 insulin syringe.

The Math Formula

Units to Pull = (Desired Dose ÷ Total mg in Vial) × mL of BAC Water Added × 100

Syringe Scale

On a standard U-100 insulin syringe, 1 mL = 100 Units. Each small tick mark is usually 1 or 2 units depending on syringe size (0.5 mL vs 1.0 mL). Always verify your syringe markings before drawing.

The Slow Drip

Always aim the BAC water down the inside glass wall of the vial. Let it dissolve passively. Never shake the vial, as this can denature the peptide and reduce efficacy.


1. Weight Loss & Metabolic Peptides

Tirzepatide — Incretin Mimetic
Vial Sizes: 10 mg / 20 mg / 30 mg
BAC Water: 2.0 mL / 2.0 mL / 3.0 mL
Route: Sub-Q
ProtocolDoseFrequencyPull (10 mg vial)Pull (20/30 mg vial)
Starting (Weeks 1–4)2.5 mgOnce weekly50 Units25 Units
Escalated (Weeks 5+)5.0 mgOnce weekly100 Units50 Units
Clinical Goal: Appetite suppression and delayed gastric emptying.
Retatrutide — Triple Agonist
Vial Sizes: 5 mg / 30 mg
BAC Water: 1.0 mL / 3.0 mL
Route: Sub-Q
ProtocolDoseFrequencyPull (5 mg vial)Pull (30 mg vial)
Starting2.0 mgOnce weekly40 Units20 Units
Maintenance4.0 mgOnce weekly80 Units40 Units
Clinical Goal: Accelerated metabolic output via GLP-1, GIP, and Glucagon receptor pathways.
AOD-9604 — Lipolytic Fragment
Vial Size: 5 mg
BAC Water: 2.0 mL (200 Units)
Route: Sub-Q
ProtocolDoseFrequencyPull
Standard300 mcg (0.3 mg)Once daily, AM on empty stomach12 Units
Clinical Goal: Targeted fat breakdown without impacting blood glucose profiles.
Tesamorelin — Growth Hormone Releasing Hormone
Vial Sizes: 2 mg / 5 mg / 10 mg
BAC Water: 1.0 mL / 2.5 mL / 5.0 mL
Route: Sub-Q
ProtocolDoseFrequencyPull (2 mg vial)Pull (5 mg vial)Pull (10 mg vial)
Standard2 mgOnce daily, 5 on / 2 off, before bed (≥2 hrs post-meal)100 Units (full vial)100 Units100 Units
Clinical Goal: Reduction of visceral, deep abdominal adipose tissue.
Fat Blaster / Lipo C — Lipotropic Compound
Vial Size: 10 mL or 30 mL pre-mixed liquid
BAC Water: None — arrives ready to use
Route: IM
ProtocolDoseFrequencyPull
Standard1.0 mL1–2 times per week100 Units (use IM needle, e.g. 25G 1")
Clinical Goal: Liver detoxification and mobilized fatty acid transport.

2. Cellular Energy & Repair Peptides

BPC-157 — Body Protection Compound
Vial Sizes: 5 mg / 10 mg
BAC Water: 2.0 mL / 4.0 mL
Route: Sub-Q
ProtocolDoseFrequencyPull (5 mg vial)Pull (10 mg vial)
Standard Healing250 mcg (0.25 mg)Twice daily (500 mcg/day total)10 Units10 Units
Clinical Goal: Systemic tissue repair, soft tissue (tendon/ligament) healing, and gut health.
KPV — Anti-inflammatory Peptide
Vial Size: 10 mg
BAC Water: 2.0 mL (200 Units)
Route: Sub-Q
ProtocolDoseFrequencyPull
Standard Range300–500 mcgOnce daily6 Units (300 mcg) / 10 Units (500 mcg)
Clinical Goal: Mast cell stabilization and lowering systemic GI/skin inflammation.
SS-31 — Mitochondrial Target
Vial Sizes: 10 mg / 50 mg
BAC Water: 2.0 mL / 10.0 mL
Route: Sub-Q
ProtocolDoseFrequencyPull (10 mg vial)Pull (50 mg vial)
Loading (4–6 week cycle)2.0 mgOnce daily40 Units40 Units
Clinical Goal: Restoring ATP production efficiency at the inner mitochondrial membrane layer.
MOTS-C — Mitochondrial Open Reading Frame
Vial Size: 10 mg
BAC Water: 1.0 mL (100 Units)
Route: Sub-Q
ProtocolDoseFrequencyPull
Standard5.0 mg (half vial)2–3 times per week50 Units
Clinical Goal: Enhancing metabolic flexibility, mimicking exercise cellular signaling, and improving carbohydrate tolerance.

3. Anti-Aging, Longevity & Skin Peptides

Glow70 / Klow Stacks — Copper Peptide Complex
Vial Sizes: 50 mg / 80 mg combined lyophilized powder
BAC Water: 2.0 mL / 3.2 mL — solution turns royal blue instantly
Route: Sub-Q
ProtocolDoseFrequencyPull (50 mg vial)Pull (80 mg vial)
Regenerative / Anti-Aging2.5 mgOnce daily10 Units10 Units
Clinical Goal: Advanced skin elasticity, structural collagen up-regulation, and accelerated skin cell turnover.
GHK-Cu — Standalone Copper Peptide
Vial Sizes: 50 mg / 100 mg
BAC Water: 2.0 mL / 4.0 mL
Route: Sub-Q
ProtocolDoseFrequencyPull (50 mg vial)Pull (100 mg vial)
Standard2.5 mgOnce daily10 Units10 Units
Note: GHK-Cu frequently causes a deep sting upon injection. To mitigate: draw your 10 Units of peptide first, then pull an extra 10–15 Units of pure BAC water into the same syringe as a local buffer before plunging.
Clinical Goal: Tissue remodeling, hair density enhancement, and wrinkle depth reduction.
Epithalon — Telomerase Activator
Vial Size: 10 mg
BAC Water: 2.0 mL (200 Units)
Route: Sub-Q
ProtocolDoseFrequencyPull
Longevity Cycle1.0 mgOnce daily before bed, 10–20 days, twice a year max20 Units
Clinical Goal: Normalizing biological clock rhythms and supporting cellular senescence delay.
Glutathione — Master Antioxidant
Vial Sizes: 1500 mg / 2000 mg powder
BAC Water: 7.5 mL / 10 mL (creates 200 mg/mL concentration)
Route: Deep IM
ProtocolDoseFrequencyPull (1500 mg vial)Pull (2000 mg vial)
Standard200 mg1–2 times per week100 Units (1.0 mL) IM100 Units (1.0 mL) IM
Clinical Goal: Oxidative stress mitigation, liver pathway clearance, and systemic skin brightening.
Snap-8 — Octapeptide (Topical Only)
DO NOT INJECT. Snap-8 is a cosmetic-grade topical peptide only.

Reconstitution: If purchased raw, dissolve into a sterile water base or mix directly into a hyaluronic acid carrier serum to form a 3% to 10% total concentration.

Application: Apply 4–6 drops topically to expression areas (crow's feet, forehead lines) twice daily.

Clinical Goal: SNARE complex disruption to topically minimize micro-muscle contractions (Botox alternative).

Master Volume & Pull Cheat Sheet

Peptide Vial Size BAC Water Added Target Dose Syringe Pull (U-100 Insulin)
Tirzepatide10 mg2.0 mL2.5 mg50 Units
Tirzepatide20 mg2.0 mL2.5 mg25 Units
Tirzepatide30 mg3.0 mL2.5 mg25 Units
Retatrutide5 mg1.0 mL2.0 mg40 Units
Retatrutide30 mg3.0 mL2.0 mg20 Units
AOD-96045 mg2.0 mL300 mcg12 Units
Tesamorelin2 mg1.0 mL2.0 mg100 Units (Full Syringe)
Tesamorelin5 mg2.5 mL2.0 mg100 Units
Tesamorelin10 mg5.0 mL2.0 mg100 Units
Fat BlasterLiquidReady1.0 mL100 Units (Use IM needle)
BPC-1575 mg2.0 mL250 mcg10 Units
BPC-15710 mg4.0 mL250 mcg10 Units
KPV10 mg2.0 mL300 mcg6 Units
SS-3110 mg2.0 mL2.0 mg40 Units
SS-3150 mg10.0 mL2.0 mg40 Units
MOTS-C10 mg1.0 mL5.0 mg50 Units
Glow / Klow50 mg2.0 mL2.5 mg10 Units
Glow / Klow80 mg3.2 mL2.5 mg10 Units
GHK-Cu50 mg2.0 mL2.5 mg10 Units
GHK-Cu100 mg4.0 mL2.5 mg10 Units
Epithalon10 mg2.0 mL1.0 mg20 Units
Glutathione1500 mg7.5 mL200 mg100 Units (Use IM needle)
Glutathione2000 mg10.0 mL200 mg100 Units (Use IM needle)
Research Use Only

All dosing information is provided for reference purposes only. Peptides are intended for laboratory research. Always follow institutional guidelines and consult relevant literature before conducting research.